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Example reconstruction work: Le et al., 2022 (Brain2Pix)
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Klenerman and Balasubramanian’s great insight was that template DNA could be sequenced by synthesizing a complementary strand of reversible terminators; basically, extending the chain one base at a time and determining the identity of each nucleotide by looking at the color of its fluorophore. In 1998, the pair started a company called Solexa to develop the technology.