It’s also worth noting that even if alternatives superior to agar were found, scientists are reluctant to abandon established protocols (even when microbiologists do use other jellies, they often still add agar to the mix, for example, to increase the gel strength of the solid media). As agar has been the standard gelling agent in microbiology for around 150 years, an enormous infrastructure of standardized methods, reference values, and quality control procedures has emerged around its specific properties. Switching to a different medium (even a superior one) means results may not be directly comparable to decades of published literature or to other laboratories’ findings.
An creates a completely separate browsing context with its own window object, its own document, and most importantly;its own prototype chain. A function hooked on HTMLMediaElement.prototype in the parent window is not the same object as HTMLMediaElement.prototype in the iframe’s window. They’re entirely separate objects. If fermaw’s audio element lived inside an iframe, my prototype hook in the parent window would never fire.
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